房水作为成纤维细胞培养中生长的调节剂。

Aqueous humor as a modulator of growth in fibroblast cultures.

作者信息

Burke J, Foster S, Herschler J

出版信息

Curr Eye Res. 1982;2(12):835-41. doi: 10.3109/02713688209020019.

DOI:10.3109/02713688209020019

Abstract

Samples of aqueous humor from rabbits and humans were examined for growth effects on subconfluent monolayer fibroblast cultures from ocular and extraocular tissues. At a concentration of 20% aqueous by medium volume, both homologous and heterologous aqueous stimulated DNA synthesis in fibroblasts from both species. The stimulation was independent of medium serum. When fibroblasts were exposed to 0 to 100% aqueous in serum-free medium or 0 to 98% aqueous in 2% serum-supplemented medium, no concentration of aqueous was observed in which DNA synthesis was inhibited. However, higher concentrations of aqueous led to increasing cell loss resulting in an apparent net inhibition of culture growth. Net growth effects of aqueous humor were, therefore, dependent upon aqueous concentration and were apparently due to the combined effects of stimulation of proliferation and reduction in cell survival.

摘要

对来自兔子和人类的房水样本进行检测，观察其对来自眼组织和眼外组织的亚汇合单层成纤维细胞培养物的生长影响。当房水在培养基中的浓度为20%（按体积计）时，同源和异源房水均能刺激两种物种成纤维细胞中的DNA合成。这种刺激与培养基中的血清无关。当成纤维细胞在无血清培养基中暴露于0%至100%的房水，或在含2%血清的培养基中暴露于0%至98%的房水时，未观察到有抑制DNA合成的房水浓度。然而，较高浓度的房水会导致细胞损失增加，从而明显对培养物生长产生净抑制作用。因此，房水的净生长效应取决于房水浓度，显然是增殖刺激和细胞存活率降低共同作用的结果。