通过免疫电子显微镜观察pBR322 V形式中的Z序列。
Visualization of Z sequences in form V of pBR322 by immuno-electron microscopy.
作者信息
Lang M C, Malfoy B, Freund A M, Daune M, Leng M
出版信息
EMBO J. 1982;1(10):1149-53. doi: 10.1002/j.1460-2075.1982.tb00005.x.
Abstract
Form V DNA has been prepared from pBR322 DNA by annealing covalently closed complementary single strands. Specific rabbit antibodies to Z-DNA were shown by radioimmunoassay and electron microscopy to react with form V DNA of pBR322. The bound antibodies were visualized either directly (on synthetic polynucleotides in Z-form), or after reaction with goat anti-rabbit immunoglobulin labeled with ferritin (on form V DNA).
摘要
通过使共价闭合的互补单链退火，从pBR322 DNA制备了V型DNA。通过放射免疫测定和电子显微镜显示，针对Z-DNA的特异性兔抗体与pBR322的V型DNA发生反应。结合的抗体可以直接观察到（在Z型合成多核苷酸上），或者在与用铁蛋白标记的山羊抗兔免疫球蛋白反应后观察到（在V型DNA上）。
Zotero 插件