Tourtellotte W W, Potvin A R, Fleming J O, Murthy K N, Levy J, Syndulko K, Potvin J H
Neurology. 1980 Mar;30(3):240-4. doi: 10.1212/wnl.30.3.240.
An empirical formula has been developed to calculate the de novo rate of synthesis of IgG in the central nervous system (CNS), based on physiologic principles that govern the passage of albumin and IgG across the blood-brain barrier (BBB). To validate the formula, radiolabeled IgG and albumin from pooled normal sera were followed from the blood to the cerebrospinal fluid (CSF) over 21 days in nine patients with definite multiple sclerosis (MS). IgG synthesis rates were calculated by the isotope exchange method and compared to values obtained with the empirical formula. There was excellent concordance, from a low rate of synthesis of 5 mg per day to a high rate of 120 mg per day. A double radiolabeled IgG experiment in two patients showed that the MS BBB processed normal serum IgG in the same way as IgG derived from autologous MS serum. Accordingly, the empirical formula, which requires only one sample of CSF and matched serum, can reliably and validly estimate the de novo rate of IgG synthesis in CNS of patients with MS.
基于白蛋白和免疫球蛋白G（IgG）通过血脑屏障（BBB）的生理原理，已开发出一种经验公式来计算中枢神经系统（CNS）中IgG的从头合成速率。为验证该公式，在9例确诊为多发性硬化症（MS）的患者中，对来自混合正常血清的放射性标记IgG和白蛋白在21天内从血液至脑脊液（CSF）进行了追踪。通过同位素交换法计算IgG合成速率，并与用经验公式获得的值进行比较。从每天5毫克的低合成速率到每天120毫克的高合成速率，两者具有极好的一致性。在两名患者中进行的双放射性标记IgG实验表明，MS的BBB处理正常血清IgG的方式与源自自体MS血清的IgG相同。因此，仅需一份CSF和匹配血清样本的经验公式，能够可靠且有效地估计MS患者CNS中IgG的从头合成速率。
