Maat J, Lupker-Wille H S
Biochim Biophys Acta. 1978 Oct 24;520(3):512-20. doi: 10.1016/0005-2787(78)90137-5.
Adenovirus type 5 (Ad5) DNA was degraded with endo R . HpaI; the left-terminal fragment, HpaI-E has recently been shown to be the smallest segment of Ad5 DNA, that can transform non-permissive cells. This fragment was labelled at its termini by limited exonuclease III digestion followed by repair synthesis with DNA polymerase and alpha-32P-labelled deoxynucleoside triphosphates. It was then further digested with each of the restriction endonucleases HpaII, HaeIII, AluI, HinfI and TaqI; the cleavage products thus obtained were ordered into a physical map.
5型腺病毒（Ad5）DNA用内切酶R.HpaI进行降解；左末端片段HpaI-E最近已被证明是Ad5 DNA中能够转化非允许细胞的最小片段。通过有限的核酸外切酶III消化，然后用DNA聚合酶和α-32P标记的脱氧核苷三磷酸进行修复合成，使该片段在其末端进行标记。然后用限制性内切酶HpaII、HaeIII、AluI、HinfI和TaqI分别对其进一步消化；将由此获得的切割产物排列成物理图谱。
