5型腺病毒DNA转化性HindIII - G片段的核苷酸序列。图谱位置4.5（HpaI位点）和8.0（HindIII位点）之间的区域。

The nucleotide sequence of the transforming HindIII-G fragment of adenovirus type 5 DNA. The region between map positions 4.5 (HpaI site) and 8.0 (HindIII site).

作者信息

Maat J, Van Ormondt H

出版信息

Gene. 1979 May;6(1):75-90. doi: 10.1016/0378-1119(79)90086-6.

Abstract

The nucleotide sequence of the region between map positions 4.5 (HpaI-site) and 8.0 (HindIII-site) of adenovirus type 5 (Ad5) DNA has been determined. This stretch of DNA is part of the transforming HindIII-G fragment, which is 2809 nucleotides long. The sequenced segment was found to have a long open reading frame for protein biosynthesis, starting 23 nucleotides from the HpaI site and extending all the way to the HindIII-G site, which could code for a protein of at least 44 000 daltons. The possible correlation beteen the coding capacity of the HindIII-G fragment and the "transforming" proteins specified by it will be discussed in the light of the recent data on the splicing of early mRNAs.

摘要

已确定5型腺病毒（Ad5）DNA图谱位置4.5（HpaI位点）和8.0（HindIII位点）之间区域的核苷酸序列。这段DNA是转化性HindIII-G片段的一部分，该片段长2809个核苷酸。发现测序的片段有一个用于蛋白质生物合成的长开放阅读框，从HpaI位点起始23个核苷酸，一直延伸到HindIII-G位点，它可能编码一种至少44000道尔顿的蛋白质。将根据最近关于早期mRNA剪接的数据，讨论HindIII-G片段的编码能力与其所指定的“转化”蛋白之间可能的相关性。