冲浪蛤蜊卵母细胞减数分裂纺锤体中微管相关蛋白的鉴定。
Identification of microtubule-associated proteins in the meiotic spindle of surf clam oocytes.
作者信息
Murphy D B
出版信息
J Cell Biol. 1980 Feb;84(2):235-45. doi: 10.1083/jcb.84.2.235.
Abstract
Meiotic spindles isolated from surf clam oocytes to morphological purity are biochemically complex, consisting of many polypeptides. These proteins fall into two classes: (a) polypeptides that are apparently cytoplasmic proteins and are not specifically associated with the spindle; and (b) polypeptides that are specifically associated with the spindle. A subset of the spindle-associated proteins, including a 250,000 mol wt component, remain with spindle tubulin through cycles of cold depolymerization and warm polymerization, showing that they are microtubule-associated proteins.
摘要
从海蛤卵母细胞中分离出的形态学上纯净的减数分裂纺锤体在生化方面是复杂的，由许多多肽组成。这些蛋白质分为两类：（a）明显是细胞质蛋白且与纺锤体无特异性关联的多肽；（b）与纺锤体特异性关联的多肽。纺锤体相关蛋白的一个子集，包括一个分子量为250,000的组分，在冷解聚和温聚合循环中与纺锤体微管蛋白保持在一起，表明它们是微管相关蛋白。
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