Geisler R W, Roggeveen A E, Hansen R J
Biochim Biophys Acta. 1978 Dec 1;544(2):284-93. doi: 10.1016/0304-4165(78)90097-1.
The effects of insulin on the turnover of glucose-6-phosphate dehydrogenase in rat epididymal adipose tissue were studied by immunochemical technique in in vitro incubations. Insulin increased the relative rate of synthesis of glucose-6-phosphate dehydrogenase by two-fold in tissue obtained from normal rats. Insulin also had an effect on the rate of degradation of this enzyme. In the absence of insulin in the incubation medium the rate constant of degradation was 0.11 h-1 (half-life, 6.3 h). When insulin was added to the medium degradation of this enzyme was slowed. The new rate constant of degradation was 0.04 h-1 (half-life, 17 h). In the presence of insulin, the rate constant of degradation of total protein in adipose tissue was unchanged; therefore the effects of insulin on the degradation of glucose-6-phosphate dehydrogenase are specific to that protein and perhaps to a few other specific proteins.
采用免疫化学技术，在体外培养条件下研究了胰岛素对大鼠附睾脂肪组织中葡萄糖-6-磷酸脱氢酶周转的影响。胰岛素使正常大鼠组织中葡萄糖-6-磷酸脱氢酶的相对合成速率提高了两倍。胰岛素对该酶的降解速率也有影响。在孵育培养基中无胰岛素时，降解速率常数为0.11 h⁻¹（半衰期为6.3小时）。当向培养基中添加胰岛素时，该酶的降解减缓。新的降解速率常数为0.04 h⁻¹（半衰期为17小时）。在有胰岛素存在的情况下，脂肪组织中总蛋白的降解速率常数不变；因此，胰岛素对葡萄糖-6-磷酸脱氢酶降解的影响是该蛋白特有的，也许对其他一些特定蛋白也是如此。
