Hafner G S, Hammond-Scoltis G, Tokarski T
Cell Tissue Res. 1980;206(2):319-32. doi: 10.1007/BF00232775.
The effect of illumination on the degradation of microvillar membrane in the invertebrate photoreceptor cell has been correlated with the appearance in the cytoplasm of certain distinct lysosome-related bodies. Three types of organelles were distinguished in the retinula cell cytoplasm of the crayfish, multivesicular bodies (MVB), both large (.20-1.50 micron) and small (1.49-0.30 micron), combination bodies (CB), and lamellar bodies (LB). Under diurnal lighting conditions significant temporal differences were found in the appearance of these three classes of organelles in the retinula cell. Small MVB are present at a consistent level throughout most of the diurnal cycle but show peak numbers at 30 min after light onset and again after 6 h of dark adaptation. Large MVB increase significantly 1 h after light onset and remain elevated through 4 h in the light. After 4 h the large MVB decline gradually for the remaining light period. Combination bodies and LB do not begin to increase until 1 h after light onset and are at peak levels between 4 and 6 h into the light period. The minimum rhabdome diameter coincides with the peak levels of large MVB, CB, and LB. These data support support the hypothesis that light causes microvillar membrane breakdown, resulting in the initial production of MVB which in turn undergo degradation to form CB and finally LB. This primary degradative response appears to be completed within the first 8 h of the light period.
光照对无脊椎动物光感受器细胞微绒毛膜降解的影响已与某些独特的溶酶体相关小体在细胞质中的出现相关联。在小龙虾的视小杆细胞细胞质中区分出了三种细胞器，多囊泡体（MVB），包括大的（0.20 - 1.50微米）和小的（1.49 - 0.30微米）、复合小体（CB）和板层小体（LB）。在昼夜光照条件下，在视小杆细胞中这三类细胞器的出现存在显著的时间差异。小MVB在昼夜周期的大部分时间里保持一致的水平，但在光照开始后30分钟以及暗适应6小时后数量达到峰值。大MVB在光照开始后1小时显著增加，并在光照下持续4小时保持升高。4小时后，大MVB在剩余的光照期间逐渐下降。复合小体和LB直到光照开始后1小时才开始增加，并在光照期的4至6小时达到峰值水平。最小视杆直径与大MVB、CB和LB的峰值水平一致。这些数据支持了这样的假设，即光照导致微绒毛膜破裂，导致最初产生MVB，而MVB又经历降解形成CB，最终形成LB。这种主要的降解反应似乎在光照期的前8小时内完成。
