Chizhevich E P, Shukoliukov S A, Tiurin V A, Mitsner B I, Sokolova N A
Biokhimiia. 1978 Sep;43(9):1653-9.
The rate of rhodopsin regeneration in decolorized rod outer segments ROS of pollock and ruff in the presence of exogenous 11Z-retinal is found to depend slightly on the temperature. The Arrhenius curve is linear within 0--20 degrees C and 0--30 degrees C in case of pollock and black ruff ROS respectively. The increase of the regeneration temperature above the upper limit results in both cases in the decrease of the chromophore binding rate due to the temperature denaturation of fish opsin. The rate of opsin regeneration in bovine ROS is temperature-dependent within 0--50 degrees C, the Arrhenius curves having a specific break with the temperature conversion at 12--13 degrees, which indicates a different 11Z-retinal binding rate with bovine opsin at low and high temperatures. Maximal rhodopsin regeneration temperature was observed within 5--10 min. for fish ROS and 1.5 hour for bovine ROS. Trimethylcyclohexene derivatives with a side chain of about 7 carbon atoms and 13Z-retinal competitively inhibited the rhodopsin regeneration in pollock and bovine ROS, while 13E-11, 12-dehydroretinal and all-E-retinal did not effect this process. Some peculiarities of the rhodopsin regeneration process in fish are discussed in connection with the molecular organization of a lipid phase of photoreceptor membrane and chromophore-binding region.
在存在外源性11Z - 视黄醛的情况下，发现狭鳕和黑皱鳃鲨脱色的视杆外段（ROS）中视紫红质的再生速率对温度略有依赖性。狭鳕和黑皱鳃鲨的ROS分别在0 - 20℃和0 - 30℃范围内，阿累尼乌斯曲线呈线性。在这两种情况下，再生温度高于上限都会导致发色团结合速率下降，这是由于鱼视蛋白发生温度变性所致。牛ROS中视蛋白的再生速率在0 - 50℃范围内依赖于温度，阿累尼乌斯曲线在12 - 13℃处有特定的温度转换断点，这表明低温和高温下11Z - 视黄醛与牛视蛋白的结合速率不同。狭鳕ROS在5 - 10分钟内观察到视紫红质再生温度最高，牛ROS则在1.5小时内。具有约7个碳原子侧链的三甲基环己烯衍生物和13Z - 视黄醛竞争性抑制狭鳕和牛ROS中的视紫红质再生，而13E - 11,12 - 脱氢视黄醛和全反式视黄醛对该过程无影响。结合光感受器膜脂质相和发色团结合区域的分子组织，讨论了鱼类视紫红质再生过程的一些特点。
