[Regeneration of rhodopsin in marine teleost fishes in the presence of exogenic of 11-cis-and all trans-retinals].

Regeneration of rhodopsin of the sea perch (Sebastodes schlegeli) and walleye pollock (Theragra chalcogramma) was studied in different preparations of eye after the addition of 11-cis- and all trans-retinals and at the expense of endogenic sources. It was established that the regeneration of rhodopsin in the eye cup, homogenate of retina and rod outer segments (ROS) at the expense of endogenic sources was extremely low and it was slightly forced by the addition of the pigment epithelium to the last two preparations. The binding degree of 11-cis retinal of walleye pollock ROS decreased with the increase of temperature of the incubation mixture and the increase of duration of storage of ROS at the temperature--10 degrees. Partial removing of lipids by hexane decreased the percentage of regeneration of the ROS preparation; on the other hand, the peroxidation of lipids practically did not affect the regenerative ability of fresh-obtained preparations. After solubilization of opsin by digitonin and sodium cholate, 40- and 50%-decreases of binding of 11-cis retinal were observed respectively. The digestion of ROS by papain, which caused no changes in the spectral properties of dark preparations, completely eliminated the regenerative ability of ROS. Regeneration of ROS at the presence of all trans-retinal proceeded at a very low speed. The specificity of the process of regeneration of rhodopsin in fishes is discussed.