Shukoliukov S A, Chizhevich E P, Korchagin V P, Dikarev V P
Biokhimiia. 1977 Oct;42(10):1747-54.
Regeneration of rhodopsin of the sea perch (Sebastodes schlegeli) and walleye pollock (Theragra chalcogramma) was studied in different preparations of eye after the addition of 11-cis- and all trans-retinals and at the expense of endogenic sources. It was established that the regeneration of rhodopsin in the eye cup, homogenate of retina and rod outer segments (ROS) at the expense of endogenic sources was extremely low and it was slightly forced by the addition of the pigment epithelium to the last two preparations. The binding degree of 11-cis retinal of walleye pollock ROS decreased with the increase of temperature of the incubation mixture and the increase of duration of storage of ROS at the temperature--10 degrees. Partial removing of lipids by hexane decreased the percentage of regeneration of the ROS preparation; on the other hand, the peroxidation of lipids practically did not affect the regenerative ability of fresh-obtained preparations. After solubilization of opsin by digitonin and sodium cholate, 40- and 50%-decreases of binding of 11-cis retinal were observed respectively. The digestion of ROS by papain, which caused no changes in the spectral properties of dark preparations, completely eliminated the regenerative ability of ROS. Regeneration of ROS at the presence of all trans-retinal proceeded at a very low speed. The specificity of the process of regeneration of rhodopsin in fishes is discussed.
在添加11-顺式视黄醛和全反式视黄醛后，以体内来源为代价，在不同的眼部制剂中研究了海鲈（Sebastodes schlegeli）和狭鳕鱼（Theragra chalcogramma）视紫红质的再生情况。结果表明，在眼杯、视网膜匀浆和视杆外段（ROS）中，以体内来源为代价的视紫红质再生极低，而在最后两种制剂中添加色素上皮细胞可使其略有增加。狭鳕鱼ROS中11-顺式视黄醛的结合程度随孵育混合物温度的升高以及ROS在-10℃下储存时间的延长而降低。用己烷部分去除脂质会降低ROS制剂的再生百分比；另一方面，脂质的过氧化实际上并不影响新鲜制备物的再生能力。在用洋地黄皂苷和胆酸钠溶解视蛋白后，分别观察到11-顺式视黄醛结合减少了40%和50%。用木瓜蛋白酶消化ROS，这对暗适应制剂的光谱特性没有影响，但完全消除了ROS的再生能力。在全反式视黄醛存在下，ROS的再生速度非常慢。文中讨论了鱼类视紫红质再生过程的特异性。
