Kujawa M, Karwacka H
Exp Pathol (Jena). 1980;18(6):329-35. doi: 10.1016/s0014-4908(80)80022-6.
Ultrastructural and stereological analysis of mitochondria of liver cells from rats was performed after intoxication with various doses of ethanol administered for various time periods in increasing concentrations. The following changes were observed in the cells of the experimental animals as compared with the control ones: -- a decrease of the surface area of mitochondria per unit volume of the hepatocyte, -- a decrease of the mean number of mitochondrial profiles per 100 micrometers 2 surface area of hepatocyte cross section, -- a decrease in the value of the axial ratio of mitochondrial profiles. A diminished volume fraction of the mitochondria was not observed. The above presented results show that, after ethanol poisoning, the mitochondria in hepatic cells become enlarged and rounded and their number seems to diminish. Qualitative studies demonstrated that the structure of mitochondria is frequently damaged, this consisting in injury to their membranes, formation of lamellar structures, and destruction of the system of cristae and matrix. This leads to the presence of degenerated, sometimes monstrously large mitochondria in the cells. The above described changes occurred as early as after 4 weeks of ethanol administration in increasing doses from 2.5 to 10 per cent. An increase of ethanol concentration to 42 per cent and prolongation of the period of treatment with ethanol to 12 weeks did not markedly enhance the changes.
对用不同浓度乙醇在不同时间段递增剂量给药后大鼠肝细胞线粒体进行超微结构和体视学分析。与对照动物相比，实验动物细胞出现以下变化：——单位体积肝细胞中线粒体表面积减少；——每100平方微米肝细胞横截面积中线粒体轮廓平均数量减少；——线粒体轮廓轴比数值降低。未观察到线粒体体积分数减少。上述结果表明，乙醇中毒后，肝细胞中的线粒体变大且呈圆形，其数量似乎减少。定性研究表明，线粒体结构常受损，表现为其膜损伤、形成层状结构以及嵴和基质系统破坏。这导致细胞中出现退化的、有时异常大的线粒体。上述变化早在以2.5%至10%递增剂量给予乙醇4周后就已出现。将乙醇浓度增至42%并将乙醇治疗时间延长至12周并未显著增强这些变化。
