Rorie D K, Muldoon S M, Tyce G M
Anesth Analg. 1981 Jan;60(1):21-7.
The effects of fentanyl on sympathetic nerve endings in canine coronary arteries have been examined. Measurements were made of change in endogenous norepinephrine in isolated coronary artery in the presence of fentanyl, and of the effects of fentanyl on norepinephrine release and metabolism using superfused strips of coronary arteries in which transmitter stores had been prelabeled with [3H]norepinephrine. [3H]Norepinephrine and its 3H metabolites were separated from samples of superfusate (physiologic salt solution flowing continuously over the prelabeled coronary artery) by column chromatography and measured by scintillation spectrometry. The addition of fentanyl (2 x 10(-6) and 8 x 10(-6) M) caused the overflow of total radioactivity (radiolabeled norepinephrine and radiolabeled metabolites of norepinephrine entering the superfusate) to increase significantly above basal levels in both unstimulated and electrically stimulated arteries. The increased overflow could be accounted for by a small increase in norepinephrine and a sizeable increase in 3,4-dihydroxyphenylglycol, the metabolite arising from the intraneuronal metabolism of norepinephrine. Because this metabolite is without agonist activity and the increases in norepinephrine were small, fentanyl in the concentrations studied had minimal effects on adrenergic neurotransmission in this vascular bed.
已对芬太尼对犬冠状动脉交感神经末梢的作用进行了研究。在存在芬太尼的情况下，对分离的冠状动脉中内源性去甲肾上腺素的变化进行了测量，并使用预先用[3H]去甲肾上腺素标记递质储存的冠状动脉灌流条带，研究了芬太尼对去甲肾上腺素释放和代谢的影响。通过柱色谱法从灌流液样品（连续流过预先标记的冠状动脉的生理盐溶液）中分离出[3H]去甲肾上腺素及其3H代谢产物，并通过闪烁光谱法进行测量。添加芬太尼（2×10⁻⁶和8×10⁻⁶M）导致在未刺激和电刺激的动脉中，总放射性（进入灌流液的放射性标记去甲肾上腺素和去甲肾上腺素的放射性标记代谢产物）的溢出量显著高于基础水平。溢出量的增加可归因于去甲肾上腺素的小幅增加以及3,4-二羟基苯乙二醇的大幅增加，3,4-二羟基苯乙二醇是去甲肾上腺素在神经元内代谢产生的代谢产物。由于这种代谢产物没有激动剂活性，且去甲肾上腺素的增加幅度较小，因此在所研究的浓度下，芬太尼对该血管床中的肾上腺素能神经传递影响极小。
