Bucher D J, Kharitonenkov I G, Lvov D K, Pysina T V, Lee H M
Intervirology. 1980;14(2):69-77. doi: 10.1159/000149165.
The hemagglutinin of an influenza virus isolated from a wild duck (Pintail, Anas acuta) in the USSR in 1976 had been found to be antigenically indistinguishable from the hemagglutinin of H2N2 viruses of human origin isolated in 1957. The hemagglutinins from viral preparations of the A/Anas acuta/Primorie/695/76 (H2Nav2) and A/Singapore/1/57 (H2N2) strains were purified by SDS gel chromatography as the subunits HA1 and HA2. Comparison of amino acid compositions and peptide maps of tryptic peptides containing [14C]-carboxymethylcysteine showed a striking degree of similarity between the H2 hemagglutinins.
1976年在苏联从一只野鸭(针尾鸭,绿头鸭)中分离出的流感病毒的血凝素,在抗原性上被发现与1957年分离出的人类源H2N2病毒的血凝素没有区别。A/绿头鸭/滨海边疆区/695/76(H2Nav2)和A/新加坡/1/57(H2N2)毒株病毒制剂中的血凝素通过SDS凝胶色谱法作为亚基HA1和HA2进行纯化。对含有[14C]-羧甲基半胱氨酸的胰蛋白酶肽的氨基酸组成和肽图进行比较,结果显示H2血凝素之间具有显著的相似程度。