流感病毒糖蛋白的糖基化位点：来自A/苏联（H1N1）血凝素糖蛋白的胰蛋白酶糖肽的特性分析
Glycosylation sites of influenza viral glycoproteins: characterization of tryptic glycopeptides from the A/USSR(H1N1) hemagglutinin glycoprotein.
作者信息
Basak S, Pritchard D G, Bhown A S, Compans R W
出版信息
J Virol. 1981 Feb;37(2):549-58. doi: 10.1128/JVI.37.2.549-558.1981.
Abstract
Glycosylated tryptic peptides of the hemagglutinin (HA) glycoprotein of influenza A/USSR/90/77(H1N1) virus were separated by a combination of ion-exchange chromatography and gel filtration. Seven different glycosylated tryptic peptide classes were obtained from the HA1 polypeptide, and only one glycosylated peptide was obtained from the HA2 polypeptide. Several of the tryptic fragments of HA1 and the HA2 glycopeptides were sulfated. The nature of the carbohydrate chain in each of the glycosylated tryptic peptides was determined from observations of the incorporation of different sugar precursors and susceptibility to cleavage by the enzyme endoglycosidase H and by compositional analysis by gas chromatography. Such analyses showed that three types of carbohydrate chains were present in HA1 (type I [complex], type II [high mannose], and hybrid type), whereas HA2 contained only type I oligosaccharide chains. The amino acid composition of each of the glycosylated tryptic peptides was also determined.
摘要
甲型流感病毒A/苏联/90/77（H1N1）血凝素（HA）糖蛋白的糖基化胰蛋白酶肽通过离子交换色谱和凝胶过滤相结合的方法进行分离。从HA1多肽中获得了7种不同的糖基化胰蛋白酶肽类，而从HA2多肽中仅获得了一种糖基化肽。HA1和HA2糖肽的几个胰蛋白酶片段被硫酸化。通过观察不同糖前体的掺入情况、对内切糖苷酶H切割的敏感性以及气相色谱组成分析，确定了每个糖基化胰蛋白酶肽中糖链的性质。此类分析表明，HA1中存在三种类型的糖链（I型[复合型]、II型[高甘露糖型]和杂合型），而HA2仅包含I型寡糖链。还确定了每个糖基化胰蛋白酶肽的氨基酸组成。
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