Dunn J F, Nisula B C, Rodbard D
J Clin Endocrinol Metab. 1981 Jul;53(1):58-68. doi: 10.1210/jcem-53-1-58.
This report describes a model of steroid transport in human plasma. The binding affinities of 21 endogenous steroids for both testosterone-binding globulin (TeBG) and corticosteroid-binding globulin (CBG) were determined under equilibrium conditions using a solid phase method at physiological pH and temperature. A computer program was used to solve the complex equilibrium interactions between these steroids and TeBG, CBG, and albumin. In this manner, we calculated the plasma distribution of each steroid into TeBG-bound, CBG-bound, albumin-bound, and unbound fractions in normal men, normal women during both the follicular and luteal phases of the ovarian cycle, and women during the third trimester of a normal pregnancy.
本报告描述了一种人体血浆中类固醇转运模型。在生理pH值和温度条件下，采用固相法在平衡条件下测定了21种内源性类固醇对睾酮结合球蛋白（TeBG）和皮质类固醇结合球蛋白（CBG）的结合亲和力。使用计算机程序求解这些类固醇与TeBG、CBG和白蛋白之间复杂的平衡相互作用。通过这种方式，我们计算了正常男性、处于卵巢周期卵泡期和黄体期的正常女性以及正常妊娠晚期女性中每种类固醇在TeBG结合、CBG结合、白蛋白结合和游离部分中的血浆分布情况。
