Jones C, Patterson D, Kao F T
Somatic Cell Genet. 1981 Jul;7(4):399-409. doi: 10.1007/BF01542985.
Purine-requiring Chinese hamster ovary cell auxotrophs of the complementation class ade-E were hybridized with various human cells, and hybrids were isolated under selective conditions in which the retention of the complementing gene on the human chromosome is necessary for survival. Synteny analysis in 72 primary and secondary hybrid clones using isozyme, karyotypic, and biochemical methods provides evidence for an assignment of the gene for phosphoribosylglycineamide formyltransferase (GART, EC 2.1.2.2), deficient in ade-E mutants, to human chromosome 14. The importance of this gene assignment to the development of hypotheses regarding the organization, structure, and regulation of genes involved in the same biosynthetic pathway in mammalian cells is discussed.
将互补类 ade - E 的需要嘌呤的中国仓鼠卵巢细胞营养缺陷型与各种人类细胞杂交，并在选择性条件下分离杂种细胞，在这些条件下，人类染色体上互补基因的保留对细胞存活是必需的。使用同工酶、核型和生化方法对 72 个初级和次级杂种克隆进行同线分析，为将 ade - E 突变体中缺乏的磷酸核糖甘氨酰胺甲酰基转移酶（GART，EC 2.1.2.2）基因定位于人类 14 号染色体提供了证据。讨论了这一基因定位对于形成关于哺乳动物细胞中参与同一生物合成途径的基因的组织、结构和调控的假说的重要性。
