顶头孢霉无细胞提取物中异青霉素N向青霉素N的转化
Conversion of isopenicillin N into penicillin N in cell-free extracts of Cephalosporium acremonium.
作者信息
Jayatilake G S, Huddleston J A, Abraham E P
出版信息
Biochem J. 1981 Feb 15;194(2):645-7. doi: 10.1042/bj1940645.
Abstract
In a cell-free system prepared by osmotic lysis of protoplasts of Cephalosporium acremonium, isopenicillin N is converted into penicillin N. The epimerase activity of the system is labile.
摘要
在通过顶头孢霉原生质体渗透裂解制备的无细胞体系中，异青霉素N可转化为青霉素N。该体系的差向异构酶活性不稳定。
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本文引用的文献
1
Purification and some properties of cephalosporin N, a new penicillin.
Biochem J. 1954 Sep;58(1):94-102. doi: 10.1042/bj0580094.
2
Incorporation of 3H and 14C from (6 alpha-3H)penicillin N and (10-14C,6 alpha-3H) penicillin N into deacetoxycephalosporin C.
Biochem J. 1980 Mar 15;186(3):889-95. doi: 10.1042/bj1860889.
3
Biosynthesis of penicillin N and cephalosporin C. Antibiotic production and other features of the metabolism of Cephalosporium sp.
Biochem J. 1967 Jun;103(3):877-90. doi: 10.1042/bj1030877.
4
Separation, purification and properties of beta-lactamase I and beta-lactamase II from Bacillus cereus 569/H/9.
Biochem J. 1974 Oct;143(1):115-27. doi: 10.1042/bj1430115.
5
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
Anal Biochem. 1976 May 7;72:248-54. doi: 10.1016/0003-2697(76)90527-3.
6
Synthesis of delta-(alpha-aminoadipyl)cysteinylvaline and its role in penicillin biosynthesis.
Biochem J. 1976 Sep 1;157(3):651-60. doi: 10.1042/bj1570651.
7
Cell-free conversion of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine into an antibiotic with the properties of isopenicillin N in Cephalosporium acremonium.
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8
Incorporation of 3H from delta-(L-alpha-amino (4,5-3H)adipyl)-L-cysteinyl-D-(4,4-3H)valine into isopenicillin N.
Biochem J. 1979 Nov 15;184(2):421-6. doi: 10.1042/bj1840421.
9
Synthesis of tritium-labelled isopenicillin N, penicillin N and 6-aminopenicillanic acid.
Biochem J. 1975 Dec;151(3):729-39. doi: 10.1042/bj1510729.
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