顶头孢霉突变体M-0198将异青霉素N无细胞转化为去乙酰氧基头孢菌素C
Cell-free conversion of isopenicillin N into deacetoxycephalosporin C by Cephalosporium acremonium mutant M-0198.
作者信息
Baldwin J E, Keeping J W, Singh P D, Vallejo C A
出版信息
Biochem J. 1981 Feb 15;194(2):649-51. doi: 10.1042/bj1940649.
Abstract
In a cell-free system prepared by lysis of protoplasts of Cephalosporium acremonium mutant M-0198, isopenicillin N was converted into a penicillinase-resistant material that behaved like deacetoxycephalosporin C on high-pressure liquid chromatography analysis. This activity was found to be unstable to storage at -80 degrees C; 70-80% of the activity was lost after 1 day.
摘要
在通过裂解顶头孢霉突变体M - 0198的原生质体制备的无细胞体系中，异青霉素N被转化为一种对青霉素酶有抗性的物质，在高压液相色谱分析中其行为类似于去乙酰氧基头孢菌素C。发现该活性在-80℃储存时不稳定；1天后70 - 80%的活性丧失。
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本文引用的文献
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2
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3
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