Lueders K K, Kuff E L
Nucleic Acids Res. 1981 Nov 25;9(22):5917-30. doi: 10.1093/nar/9.22.5917.
The genome of M. musculus contains many copies of DNA sequences homologous to the 35S RNA of intracisternal type-A particles (IAP) (1,2). A major class of IAP genes has been identified and isolated from a mouse library in Charon 4A (3). Cloned mouse IAP genes were used as probes to study homologous sequences in the DNA of other species. Sequences related to mouse IAP genes were detected in the DNAs from a variety of animal cells. DNAs from rat, gerbil, and hamster cells all gave strong reactions which could be localized to discrete restriction fragments on genomic blots. The reaction of Syrian hamster DNA was particularly strong. Fragments derived from different parts of the IAP gene all reacted with Syrian hamster DNA, and the reactive restriction fragments in the Syrian hamster DNA could be ordered with reference to the known restriction map of the IAP genes. The data suggest that sequences related to mouse IAP genes make up a 7 Kb unit in the Syrian hamster genome. Since the majority of the hamster sequences are quite divergent from those in the mouse, the ease with which they are detected suggests that they must be reiterated in the hamster genome.
小家鼠的基因组包含许多与A型颗粒（IAP）的35S RNA同源的DNA序列拷贝（1,2）。已从Charon 4A载体的小鼠文库中鉴定并分离出一类主要的IAP基因（3）。克隆的小鼠IAP基因被用作探针来研究其他物种DNA中的同源序列。在来自多种动物细胞的DNA中检测到与小鼠IAP基因相关的序列。来自大鼠、沙鼠和仓鼠细胞的DNA均产生强烈反应，这些反应可定位到基因组印迹上的离散限制性片段。叙利亚仓鼠DNA的反应尤为强烈。源自IAP基因不同部分的片段均与叙利亚仓鼠DNA发生反应，并且叙利亚仓鼠DNA中的反应性限制性片段可参照IAP基因的已知限制性图谱进行排序。数据表明，与小鼠IAP基因相关的序列在叙利亚仓鼠基因组中构成一个7 Kb的单元。由于仓鼠的大多数序列与小鼠的序列差异很大，它们被检测到的容易程度表明它们在仓鼠基因组中必定是重复的。
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