尿液中胎盘雌三醇液相色谱测定方法的改进
Improved liquid chromatographic determination of placental estriol in urine.
作者信息
Gotelli G R, Wall J H, Kabra P M, Marton L J
出版信息
Clin Chem. 1978 Dec;24(12):2132-4.
PMID:719859
Abstract
Placental estriol in urine can be determined, after enzymatic hydrolysis, by liquid chromatography. A single-step extraction technique results in rapid processing with minimal sample manipulation. Analytical recovery of estriol added to urine was 96 to 106%. Day-to-day precision (CV), established by 10 replicate analyses at a mean concentration of 15.9 mg of estriol per liter, was 3.7%.
摘要
尿液中的胎盘雌三醇在酶水解后可用液相色谱法测定。单步萃取技术可实现快速处理，且样品处理最少。添加到尿液中的雌三醇的分析回收率为96%至106%。在每升15.9毫克雌三醇的平均浓度下进行10次重复分析所确定的日常精密度（CV）为3.7%。
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