Diedrich K, Evans S E, Gibitz H J, Helbing A R, Kynast G, Heinze T, Liedtke B, Lorenz U, Martens G, Schlebusch H, Schiøler V, Bouchelouche P, Siggaard-Andersen O, Sirowey H, Weihs D, Kessler A C, Beutler H O
Z Geburtshilfe Perinatol. 1982 Feb;186(1):19-26.
A new test-combination for the enzymatic determination of lecithin in amniotic fluid for the assessment of fetal lung maturity has been developed by Boehringer Mannheim. This test was evaluated by 12 hospitals and has been compared with the L/S ratio, the foam-test or the densitometric determination of lecithin. The assay is based on the hydrolysis of lecithin by phospholipase C which starts an enzymatic chain reaction in which NADH consumption if measured photometrically. The intra- and interassay precision were characterized by CV values below 10%. Average recoveries of lecithin were 95-102%. It is recommended to centrifuge the samples (10 min, 700 g) and to start the analysis as soon as possible after receipt of the specimen. The total amount of time required is 2 hours for a single determination. Batches of up to 10 samples require little extra time. An opened test-combination can be used for a maximum of 30 single determinations. Comparison of the quantitative enzymatic lecithin determination with other methods showed that the critical value for lecithin is 5.0 mg/100 ml. Above 5.1 mg/100 ml no case respiratory distress syndrome was observed. The good precision accuracy and the simple handling make the enzymatic lecithin determination suitable for routine use.
勃林格殷格翰公司研发了一种用于酶法测定羊水卵磷脂以评估胎儿肺成熟度的新型检测组合。12家医院对该检测进行了评估，并与卵磷脂/鞘磷脂比值、泡沫试验或卵磷脂的密度测定法进行了比较。该测定基于磷脂酶C对卵磷脂的水解，由此启动酶促连锁反应，通过光度法测定其中烟酰胺腺嘌呤二核苷酸（NADH）的消耗。批内和批间精密度以低于10%的变异系数（CV）值为特征。卵磷脂的平均回收率为95% - 102%。建议对样本进行离心（10分钟，700克），收到标本后尽快开始分析。单次测定所需总时间为2小时。一批多达10个样本所需额外时间很少。一个开封的检测组合最多可用于30次单次测定。将定量酶法测定卵磷脂与其他方法进行比较表明，卵磷脂的临界值为5.0毫克/100毫升。高于5.1毫克/100毫升时，未观察到一例呼吸窘迫综合征。良好的精密度、准确性和简便的操作使得酶法测定卵磷脂适用于常规使用。
