Heartlein M W, O'Neill J P, Pal B C, Preston R J
Mutat Res. 1982 Feb 22;92(1-2):411-6. doi: 10.1016/0027-5107(82)90239-1.
The induction of 6-thioguanine-resistant (TGr) mutants and sister-chromatid exchanges (SCE) by 5-bromo- and 5-chloro-deoxyuridine (BrdU and CldU) was determined with Chinese hamster ovary cells in culture. Over a concentration range of 0.5-200 microM, CldU treatment caused 3-5 times the number of SCEs found with BrdU; for example, with 10 microM CldU, 60.0 +/- 14.2 SCEs/metaphase (mean +/- S.D.) were induced, while with 10 microM BrdU, only 14.6 +/- 4.1 SCEs/metaphase were found. Despite this difference in SCE induction, both compounds were mutagenic only at concentrations greater than 50 microM, and BrdU was more mutagenic than CldU. These results indicate that SCEs and gene mutations are induced by these thymidine analogs through 2 different mechanisms.
在培养的中国仓鼠卵巢细胞中，测定了5-溴脱氧尿苷和5-氯脱氧尿苷（BrdU和CldU）对6-硫鸟嘌呤抗性（TGr）突变体和姐妹染色单体交换（SCE）的诱导作用。在0.5-200 microM的浓度范围内，CldU处理诱导的SCE数量是BrdU的3-5倍；例如，用10 microM CldU处理时，中期细胞诱导出60.0±14.2个SCE/中期（平均值±标准差），而用10 microM BrdU处理时，仅发现14.6±4.1个SCE/中期。尽管在SCE诱导方面存在这种差异，但两种化合物仅在浓度大于50 microM时才具有致突变性，且BrdU的致突变性比CldU更强。这些结果表明，这些胸苷类似物通过两种不同机制诱导SCE和基因突变。
