Dahrenmöller N, Roessner A, Pott G, Müller K M
Microsc Acta Suppl. 1979(3):191-6.
50 liver specimens with fibrotic liver disease were taken from current autopsy material and, after preparation, subjected to automatized image analysis by QUANTIMET 720 for quantitation of the connective tissues and their cellularity. In cell-free supernatants of liver tissue homogenates we determined the activities of N-acetyl-beta-glucosaminidase (beta-NAG, E.C. 3.2.1.30), a lysosomal glycosidase that is involved in proteoglycane metabolism of connective tissue. Beta-NAG-isoenzymes were demonstrated by electrofocusing in thin layers of polyamide gel. The results showed statistically significant correlations between beta-NAG activity and morphometrically assessed quantities of connective tissue, and of mesenchymal cells found in the latter or in the whole specimen, respectively. Demonstration of tissue or serum levels of beta-NAG or its isoenzymes provides a readily practicable method for assessing mesenchymal activities of the liver.
从当前尸检材料中获取50份患有肝纤维化疾病的肝脏标本，制备后用QUANTIMET 720进行自动化图像分析，以定量结缔组织及其细胞成分。在肝脏组织匀浆的无细胞上清液中，我们测定了N-乙酰-β-氨基葡萄糖苷酶（β-NAG，E.C. 3.2.1.30）的活性，这是一种参与结缔组织蛋白聚糖代谢的溶酶体糖苷酶。通过在聚酰胺凝胶薄层中进行等电聚焦来显示β-NAG同工酶。结果显示，β-NAG活性与形态学评估的结缔组织数量以及分别在结缔组织或整个标本中发现的间充质细胞数量之间存在统计学上的显著相关性。检测β-NAG或其同工酶的组织或血清水平为评估肝脏的间充质活性提供了一种易于实践的方法。
