Feyerabend C, Levitt T, Russell M A
J Pharm Pharmacol. 1975 Jun;27(6):433-6.
A rapid gas-liquid chromatographic method for the estimation of nicotine in plasma is described. Nicotine is extracted from alkalinized plasma into diethyl ether. This is then concentrated by evaporation and, after an acid back extraction is re-extracted into n-heptane (nitrogen detector) or dichloromethane (flame ionization detector) before injection onto the gas chromatography. Thirty samples a day can be analysed by this method which enables concentrations of 0.1 ng ml- minus 1 nicotine to be measured. It is thus possible to measure nicotine in plasma and urine samples from non-smokers.
本文描述了一种用于估算血浆中尼古丁含量的快速气-液色谱法。尼古丁从碱化血浆中萃取到乙醚中。然后通过蒸发进行浓缩，经过酸反萃取后，再萃取到正庚烷（氮检测器）或二氯甲烷（火焰离子化检测器）中，然后注入气相色谱仪。该方法每天可分析30个样本，能够测量低至0.1 ng/ml的尼古丁浓度。因此，有可能测量非吸烟者血浆和尿液样本中的尼古丁含量。
