在低离子强度下从核小体中选择性去除组蛋白H1。
Selective removal of histone H1 from nucleosomes at low ionic strength.
作者信息
Modak S P, Lawrence J J, Gorka C
出版信息
Mol Biol Rep. 1980 Dec 31;6(4):235-43. doi: 10.1007/BF00777531.
The method for removal of histone H 1 from chromatin by treatment with ion-exchange resin AG 50 WX 2 in the presence of 100 mM NaCl and 50 mM phosphate buffer (Thoma and Koller, 1977, Cell, 12, 101-107) results in production not only of H1-depleted chromatin but also free DNA. We have not modified this procedure so that the nucleosome is treated with the cation exchange resin in two steps, first in 50 mM sodium phosphate buffer and then in 50 mM sodium phosphate and 50 mM NaCl whereby histone H 1 is selectively removed without a release of free DNA at low resin concentrations.
在100 mM氯化钠和50 mM磷酸盐缓冲液存在的情况下，通过用离子交换树脂AG 50 WX 2处理从染色质中去除组蛋白H 1的方法（托马和科勒，1977年，《细胞》，第12卷，第101 - 107页）不仅会产生H1缺失的染色质，还会产生游离DNA。我们没有修改这个程序，而是将核小体分两步用阳离子交换树脂处理，首先在50 mM磷酸钠缓冲液中，然后在50 mM磷酸钠和50 mM氯化钠中，这样在低树脂浓度下可以选择性地去除组蛋白H 1而不释放游离DNA。
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