组蛋白H1对染色质结构的影响。

Influence of histone H1 on chromatin structure.

作者信息

Thoma F, Koller T

出版信息

Cell. 1977 Sep;12(1):101-7. doi: 10.1016/0092-8674(77)90188-x.

Abstract

Removal of histone H1 produces a transition in the structure of chromatin fibers as observed by electron microscopy. Chromatin containing all histone proteins appears as fibers with a diameter of about 250 A. The nucleosomes within these fibers are closely packed. If histone H1 is selectively removed with 50-100 mM NaCl in 50 mM sodium phosphate buffer (pH 7.0) in the presence of the ion-exchange resin AG 50 W - X2, chromatin appears as "beads-on-a-string" with the nucleosomes separated from each other by distances of about 150-200 A. If chromatin is treated in the presence of the resin with NaCl at concentrations of 650 mM or more, the structural organization of the chromatin is decreased, yielding fibers of irregular appearance.

摘要

如通过电子显微镜观察到的，去除组蛋白H1会导致染色质纤维结构发生转变。含有所有组蛋白的染色质呈现为直径约250埃的纤维。这些纤维中的核小体紧密堆积。如果在离子交换树脂AG 50 W - X2存在的情况下，于50 mM磷酸钠缓冲液（pH 7.0）中用50 - 100 mM NaCl选择性去除组蛋白H1，染色质会呈现为“串珠”状，核小体彼此之间相隔约150 - 200埃。如果在树脂存在的情况下用浓度为650 mM或更高的NaCl处理染色质，染色质的结构组织会被破坏，产生外观不规则的纤维。