[噬菌体φ80新结构基因的定位]
[Mapping new structural genes of phage phi80].
作者信息
Rybchin V N, Furaev V V, Grigor'ev P S, Ziuzenkova E P, Kochnev I G
出版信息
Genetika. 1978 Sep;14(9):1539-42.
A new set of am and ts-mutants of the phage phi80 was isolated. A series of lambdacI857h80att80int3 prophage deletion strains, extending for various lengths into the region of structural genes was generated and characterized in order to facilitate the mapping of the phi80 am and ts-mutants. Complementation test allowed to find out 5 new genes named 20, 21, 22, 23 and 24. The result of the deletion mapping of structural genes is as follows: 1-2-3-20-4-5-6-21-7-22-9-23-10-8-12-24-13.
分离出了一组新的噬菌体φ80的am和ts突变体。构建并鉴定了一系列λcI857h80att80int3原噬菌体缺失菌株，这些菌株在结构基因区域延伸了不同长度，以便于对φ80的am和ts突变体进行定位。互补试验发现了5个新基因，分别命名为20、21、22、23和24。结构基因的缺失定位结果如下：1-2-3-20-4-5-6-21-7-22-9-23-10-8-12-24-13。
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