Pinault L, Milhaud G
Toxicol Eur Res. 1981 Jan;3(1):51-4.
The analytical method which is described enables to detect principal anticoagulants presently used in France as rodenticides. Its particularity lies in using a mixture of iso octane and dichloromethane 95/5 (V/V) in the extractive part whose output varies according to the compounds from 80 to 25 per cent in supplemented liver. Besides thin layer chromatography which is used to achieve identification and semi quantitative evaluation, spectrometric measurement can be carried through to confirm identity and precise quantity with more accuracy. Sensitivity varying according to compounds, it is possible to detect products whose minimum concentration in biological materials is between 1 and 5 ppm.
所描述的分析方法能够检测法国目前用作灭鼠剂的主要抗凝血剂。其独特之处在于在萃取部分使用异辛烷和二氯甲烷95/5（体积比）的混合物，其产率根据化合物的不同在添加肝脏中从80%到25%不等。除了用于进行鉴定和半定量评估的薄层色谱法外，还可以进行光谱测量以更准确地确认身份和精确含量。灵敏度因化合物而异，能够检测生物材料中最低浓度在1至5 ppm之间的产品。
