Crowle A J, Cline L J
J Immunol Methods. 1977;17(3-4):379-81. doi: 10.1016/0022-1759(77)90122-3.
We describe an improved immunodiffusion stain using trichloroacetic acid to mordant dying by Crocein scarlet. Background is readily cleared without over-destaining using 0.3% acetic acid.