体内暴露于化学诱变剂甲磺酸乙酯的雄性小鼠生殖细胞中的非预定DNA合成。
Unscheduled DNA synthesis in the germ cells of male mice exposed in vivo to the chemical mutagen ethyl methanesulfonate.
作者信息
Sega G A
出版信息
Proc Natl Acad Sci U S A. 1974 Dec;71(12):4955-9. doi: 10.1073/pnas.71.12.4955.
Abstract
An unscheduled DNA synthesis has been clearly demonstrated in meiotic and postmeiotic germ cell stages of the male mouse after in vivo treatment with a 250 mg/kg dose of ethyl methanesulfonate. The germ cell stages showing unscheduled DNA synthesis range from early to middle meiotic prophase stages through early to middle spermatid stages. The initiation of this synthesis, taken to be repair of chemically damaged DNA in these germ cells, is rapid, beginning within 1 hour after the injection of ethyl methanesulfonate. Unscheduled DNA synthesis has not been detected in the most mature germ cell stages, which give rise to dominant lethal mutations, nor does it occur in germ cell stages where protamine has replaced the chromosomal histones.
摘要
在用250mg/kg剂量的甲磺酸乙酯进行体内处理后，已在雄性小鼠减数分裂和减数分裂后生殖细胞阶段清楚地证明了非程序性DNA合成。显示非程序性DNA合成的生殖细胞阶段范围从减数分裂前期早期到中期，再到精细胞早期到中期。这种合成的起始，被认为是这些生殖细胞中化学损伤DNA的修复，是快速的，在注射甲磺酸乙酯后1小时内开始。在最成熟的生殖细胞阶段未检测到非程序性DNA合成，这些阶段会产生显性致死突变，在鱼精蛋白取代染色体组蛋白的生殖细胞阶段也不会发生。
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