Wintermans J F, Van Besouw A, Bögemann G
Biochim Biophys Acta. 1981 Jan 26;663(1):99-107. doi: 10.1016/0005-2760(81)90197-1.
Spinach envelopes were isolated at pH 8.5, which is inhibitory to interlipid galactosyl transfer, and at pH 7.2, which is close to values used in most recent work on chloroplast envelopes. Peptide patterns were identical, but galactolipid compositions differed markedly. These changes are such as could be explained by interlipid galactosyl transfer during isolation at pH 7.2, i.e. a strong decrease in monogalactolipid and a corresponding increase in tri- and tetragalactolipids and diacylglycerol. Digalactolipid was little affected. It was further observed that the interlipid galactosyl transfer was restricted to lipids rich in hexadecatrienoic acid. Arguments are presented that the lipid composition of envelopes in vivo is better represented by the results obtained at high pH. The results will be discussed in relation to previous data on galactolipid composition and biosynthesis in envelopes.
菠菜包膜是在pH 8.5（抑制脂质间半乳糖基转移）和pH 7.2（接近最近关于叶绿体包膜研究中使用的值）条件下分离得到的。肽谱相同，但半乳糖脂组成有显著差异。这些变化可以用在pH 7.2分离过程中的脂质间半乳糖基转移来解释，即单半乳糖脂大幅减少，三半乳糖脂、四半乳糖脂和二酰基甘油相应增加。二半乳糖脂受影响较小。进一步观察到脂质间半乳糖基转移仅限于富含十六碳三烯酸的脂质。有观点认为，高pH条件下获得的结果能更好地反映体内包膜的脂质组成。将结合先前关于包膜中半乳糖脂组成和生物合成的数据来讨论这些结果。
