Ohnaus E E, Frösch R
Hepatogastroenterology. 1980 Apr;27(2):141-5.
Many different experimental models for the induction of gastric ulcers have been reported in rats. Most of these experimental designs are often not reproducible and the interpretation of the results obtained is sometimes difficult. In the present study, three different models were found to give reliable and reproducible results, which could be repeated at any time of the year with different experimenters performing the procedure. To date, these methods are in our opinion, the best in designing gastric ulcer experiments and assessing the effect of pharmacological active substances. Two pharmacological active substances were investigated in the present study, pindolol, a beta-blocking agent with intrinsic sympathomimetic activity and salmon calcitonin, a hormone influencing calcium homeostasis in blood. Using all three different models no effect was seen after pindolol administration, while a strong inhibitory effect on the formation of gastric ulcers was observed after salmon calcitonin. Following ligation of the pylorus, the ulcer formation rate was significantly decreased from 80 to 33% with a significant fall in ulcer index from 2 to 0.42 and reduction of the ulcer areas from 6.6 to 0.58 mm2 (p less than 0.05). In addition, following phenylbutazone administration the appearance of gastric ulcers was diminished after salmon calcitonin from 1.4 to 0.43 and in ulcer area from 4.5 to 0.95 mm2 (p less than 0.01). These three ulcer models used in the present study were found to be very reliable as compared with other models reported in the literature and tested in our laboratory.
已经报道了许多在大鼠中诱导胃溃疡的不同实验模型。这些实验设计大多往往不可重复，对所得结果的解释有时也很困难。在本研究中，发现三种不同的模型能给出可靠且可重复的结果，一年中的任何时候不同实验者进行该操作都能重复。迄今为止，在我们看来，这些方法是设计胃溃疡实验和评估药理活性物质效果的最佳方法。本研究中研究了两种药理活性物质，吲哚洛尔，一种具有内在拟交感活性的β受体阻滞剂，以及鲑鱼降钙素，一种影响血液中钙稳态的激素。使用所有三种不同模型，给予吲哚洛尔后未见效果，而给予鲑鱼降钙素后观察到对胃溃疡形成有强烈抑制作用。幽门结扎后，溃疡形成率从80%显著降至33%，溃疡指数从2显著降至0.42，溃疡面积从6.6平方毫米降至0.58平方毫米（p<0.05）。此外，给予保泰松后，鲑鱼降钙素使胃溃疡的出现从1.4减少至0.43，溃疡面积从4.5平方毫米减少至0.95平方毫米（p<0.01）。与文献报道并在我们实验室测试的其他模型相比，本研究中使用的这三种溃疡模型被发现非常可靠。
