Halstead S B, Tom M C, Elm J L
Infect Immun. 1981 Jan;31(1):102-6. doi: 10.1128/iai.31.1.102-106.1981.
We wished to find a simple, biologically relevant method to evaluate the virulence of dengue viruses for human beings. Since cells of mononuclear phagocyte lineage may be important sites of dengue infection in primates, we evaluated the permissiveness of these cells to dengue virus as a correlate of virus virulence. Two wild-type, large-plaque, monkey-virulent dengue-2 virus strains and two small-plaque, monkey-avirulent dengue-2 virus strains were evaluated for their ability to replicate in human peripheral blood leukocyte cultures supplemented with enhancing antibody. One of the small-plaque strains was demonstrated to have reduced virulence for man. Wild-type dengue-2 viruses replicated readily in peripheral blood leukocyte suspension cultures, whereas small-plaque dengue-2 strains did not. Differences between our data and results obtained by other workers employing adherent peripheral blood leukocytes are discussed. Antibody-enhanced growth of dengue virus in suspension cultures of human peripheral blood leukocytes gives promise of being a simple in vitro system for characterizing dengue virus virulence.
我们希望找到一种简单的、与生物学相关的方法来评估登革病毒对人类的毒力。由于单核吞噬细胞系的细胞可能是灵长类动物登革热感染的重要部位，我们评估了这些细胞对登革病毒的易感性，将其作为病毒毒力的一个相关指标。我们评估了两株野生型、大蚀斑、对猴有毒力的登革2型病毒株和两株小蚀斑、对猴无毒力的登革2型病毒株在添加增强抗体的人外周血白细胞培养物中的复制能力。其中一株小蚀斑病毒株对人的毒力已被证明有所降低。野生型登革2型病毒能在外周血白细胞悬浮培养物中轻易复制，而小蚀斑登革2型病毒株则不能。我们讨论了我们的数据与其他研究人员使用贴壁外周血白细胞所获得结果之间的差异。登革病毒在人外周血白细胞悬浮培养物中的抗体增强生长有望成为一种简单的体外系统，用于表征登革病毒的毒力。
