Liprandi F, Walder R
Arch Virol. 1983;76(1):51-61. doi: 10.1007/BF01315703.
Virulent and attenuated strains of yellow fever virus were compared for their ability to grow in cultures of unstimulated leucocytes and monocytes derived from human peripheral blood, and of a macrophage-like cell line of human origin, U937. The extent of virus growth in leucocyte cultures varied depending on the strain of virus, multiplicity of infection, presence of diluted antibody in the culture medium but independently of the flavivirus immune status of the donor. The same pattern of differential growth was observed in the three types of cultures used. Although strain related variation in growth occurred within both virulent and attenuated strains, most of the attenuated strains produced higher virus yields than the virulent ones, suggesting that replication in this cell system is not related to the expression of virulence for the host. Replication in human monocytes as an in vitro marker of immunogenity for substrains of 17 D vaccine virus is discussed.
对黄热病病毒的强毒株和减毒株在源自人外周血的未刺激白细胞和单核细胞培养物以及人源巨噬细胞样细胞系U937中的生长能力进行了比较。白细胞培养物中病毒生长的程度因病毒株、感染复数、培养基中稀释抗体的存在而有所不同，但与供体的黄病毒免疫状态无关。在所用的三种培养类型中观察到了相同的差异生长模式。尽管在强毒株和减毒株中均出现了与毒株相关的生长差异，但大多数减毒株产生的病毒产量高于强毒株，这表明在该细胞系统中的复制与对宿主的毒力表达无关。讨论了在人单核细胞中的复制作为17D疫苗病毒亚株免疫原性的体外标志物。
