Scott R M, Nisalak A, Cheamudon U, Seridhoranakul S, Nimmannitya S
J Infect Dis. 1980 Jan;141(1):1-6. doi: 10.1093/infdis/141.1.1.
Dengue viruses were isolated by a plaque technique in LLC-MK2 cells from washed peripheral blood leukocytes of patients with hemorrhagic fever. In comparison with plasma, the use of peripheral blood leukocytes permitted greater than three times the recovery rate of viruses, allowed for the isolation of strains of virus from patients with high serum levels of antibody to dengue virus, and extended the period of detectable viremia. The use of peripheral blood leukocytes was especially useful for isolation of viruses from patients with hemorrhagic fever, in whom antibody titers were generally quite high during the acute phase of the disease. This method is recommended for use by laboratories with access to the appropriate acute-phase specimens. Of the peripheral blood leukocytes, adherent monocytes appeared to be the cells most likely affected; however, involvement of atypical lymphocytes, which are commonly found in patients with hemorrhagic fever, or polymorphonuclear leukocytes could not be excluded by this study. Only a small number of infected centers could be identified by infectious-center assays, but the marked augmentation of recovery of virus with the use of peripheral blood leukocytes indicated the presence in patients of a subpopulation of cells that permit dengue virus infection.
通过蚀斑技术，从出血热患者经洗涤的外周血白细胞中，在LLC-MK2细胞中分离出登革病毒。与血浆相比，使用外周血白细胞可使病毒回收率提高三倍以上，能够从血清中登革病毒抗体水平较高的患者中分离出病毒株，并延长了可检测到病毒血症的时间。外周血白细胞的使用对于从出血热患者中分离病毒特别有用，在这些患者中，疾病急性期的抗体滴度通常相当高。建议有条件获取适当急性期标本的实验室采用这种方法。在外周血白细胞中，贴壁单核细胞似乎是最易受影响的细胞；然而，本研究不能排除出血热患者中常见的非典型淋巴细胞或多形核白细胞受到感染。通过感染中心测定只能识别少数感染中心，但使用外周血白细胞后病毒回收率显著提高，表明患者体内存在允许登革病毒感染的细胞亚群。
