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从在小鼠中引发脆弱拟杆菌纯感染开始就使用半固体琼脂。

Use of semisolid agar from initiation of pure Bacteroides fragilis infection in mice.

作者信息

Walker C B, Wilkins T D

出版信息

Infect Immun. 1976 Sep;14(3):721-5. doi: 10.1128/iai.14.3.721-725.1976.

DOI:10.1128/iai.14.3.721-725.1976
PMID:965094
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC420946/
Abstract

The development of a pure Bacteroides fragilis infection in mice is described. The infection produces large subcutaneous abscesses at the site of injection which can be observed grossly within 7 days after injection. The infection was initiated by infection of pure cultures grown in semisolid agar medium. Similar infections were also produced with pure cultures of B. distasonis, B. ovatus, B. thetaiotaomicron, and B. vulgatus. However, a distinct deoxyribonucleic acid homology group, formerly classified as B. thetaiotaomicron, did not produce abscesses in any of the mice tested.

摘要

本文描述了小鼠中脆弱拟杆菌纯感染的发展情况。该感染在注射部位产生大的皮下脓肿,在注射后7天内可肉眼观察到。感染由在半固体琼脂培养基中生长的纯培养物感染引发。用解氏拟杆菌、卵形拟杆菌、多形拟杆菌和普通拟杆菌的纯培养物也产生了类似的感染。然而,一个以前被归类为多形拟杆菌的独特脱氧核糖核酸同源组在任何测试小鼠中都未产生脓肿。

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本文引用的文献

1
Liver abscess production by non-spore-forming anaerobic bacteria in a mouse model.非产芽孢厌氧菌在小鼠模型中引发肝脓肿
Infect Immun. 1974 Mar;9(3):599-603. doi: 10.1128/iai.9.3.599-603.1974.
2
Use of anaerobic glove boxes for the cultivation of strictly anaerobic bacteria.使用厌氧手套箱培养严格厌氧菌。
Am J Clin Nutr. 1972 Dec;25(12):1329-34. doi: 10.1093/ajcn/25.12.1329.
3
Micromethod for identification of anaerobic bacteria: design and operation of apparatus.厌氧细菌鉴定的微量方法:仪器的设计与操作
Appl Microbiol. 1975 Nov;30(5):831-7. doi: 10.1128/am.30.5.831-837.1975.
4
Development of a micromethod for identification of anaerobic bacteria.一种用于鉴定厌氧菌的微量方法的开发。
Appl Microbiol. 1975 Nov;30(5):825-30. doi: 10.1128/am.30.5.825-830.1975.
5
In vivo protection of Fusobacterium necrophorum from penicillin by Bacteroides fragilis.脆弱拟杆菌对坏死梭杆菌在体内的青霉素保护作用。
Antimicrob Agents Chemother. 1975 May;7(5):698-703. doi: 10.1128/AAC.7.5.698.