Lubaroff D M
J Urol. 1977 Oct;118(4):612-5. doi: 10.1016/s0022-5347(17)58123-9.
The development of a tissue culture line of human prostatic adenocarcinoma is described. The culture (HPC-36), derived from tumor tissue explants, is a pure epithelial culture with characteristics of neoplastic cells, including a large nuclear size relative to the amount of cytoplasm, multiple nucleoli within the nucleus, many mitotic figures, the formation of multinucleated giant cells and the loss of contact inhibition. The cells also stained positively for acid phosphatase and have been grown in monolayer and suspension cultures. The HPC-36 cells presently are being studied to determine whether they are true descendants of the cancer cells.
本文描述了人前列腺腺癌组织培养系的建立。该培养物（HPC - 36）源自肿瘤组织外植体，是一种具有肿瘤细胞特征的纯上皮培养物，包括细胞核相对于细胞质体积较大、核内有多个核仁、有许多有丝分裂象、形成多核巨细胞以及失去接触抑制。这些细胞酸性磷酸酶染色呈阳性，已在单层培养和悬浮培养中生长。目前正在对HPC - 36细胞进行研究，以确定它们是否为癌细胞的真正后代。
