Oxlund H, Andreassen T T
J Anat. 1980 Dec;131(Pt 4):611-20.
Biomechanical testing of connective tissue is usually done on whole tissue. Studies of the mechanical properties of each component of the tissue, however, are important for elucidating the connection between the functional properties and the molecular structure. Enzymic degradation of one component at a time followed by mechanical testing was employed. The specificity of enzymes used was ascertained with mechanical tests on collagen films from purified, reconstituted collagen after enzymic treatment. The study shows that collagen is the component which is mainly responsible for the tensile strength of skin and aorta, while the toe-part of the 'stress'-strain curve of aorta is determined by both elastin and collagen. The hyaluronic acid does not seem to play any role in the static mechanical properties of skin and aorta as evaluated by means of the present method.
结缔组织的生物力学测试通常在整个组织上进行。然而，研究组织各成分的力学性能对于阐明功能特性与分子结构之间的联系很重要。采用一次酶解一种成分然后进行力学测试的方法。通过对酶处理后纯化、重组胶原蛋白制成的胶原膜进行力学测试，确定了所用酶的特异性。研究表明，胶原蛋白是主要负责皮肤和主动脉抗张强度的成分，而主动脉“应力”-应变曲线的趾部由弹性蛋白和胶原蛋白共同决定。就目前所采用的方法评估而言，透明质酸似乎在皮肤和主动脉的静态力学性能中不起任何作用。
