Groseclose E E, Ribbons D W
J Bacteriol. 1981 May;146(2):460-6. doi: 10.1128/jb.146.2.460-466.1981.
We present evidence to document a third pathway for the microbial catabolism of resorcinol. Resorcinol is converted to pyrogallol by resorcinol-grown cells of Azotobacter vinelandii. Pyrogallol is the substrate for one of two ring cleavage enzymes induced by growth with resorcinol. Oxalocrotonate, CO2, pyruvate, and acetaldehyde have been identified as products of pyrogallol oxidation catalyzed by extracts of resorcinol-grown cells. The enzymes pyrogallol 1,2-dioxygenase, oxalocrotonate tautomerase (isomerase), oxalocrotonate decarboxylase, and vinylpyruvate hydratase are present in extracts from resorcinol-grown cells but not in succinate-grown cells.
我们提供证据证明间苯二酚微生物分解代谢的第三条途径。间苯二酚可被维涅兰德固氮菌中以间苯二酚为生长底物的细胞转化为连苯三酚。连苯三酚是由间苯二酚培养诱导产生的两种环裂解酶之一的底物。草酰巴豆酸、二氧化碳、丙酮酸和乙醛已被鉴定为间苯二酚培养细胞提取物催化连苯三酚氧化的产物。连苯三酚1,2 - 双加氧酶、草酰巴豆酸互变异构酶（异构酶）、草酰巴豆酸脱羧酶和乙烯基丙酮酸水合酶存在于间苯二酚培养细胞的提取物中，而不存在于琥珀酸培养细胞的提取物中。
