Grover B G, Sharma S C
J Comp Neurol. 1981 Mar 1;196(3):471-88. doi: 10.1002/cne.901960310.
Nonretinal sources of tectal afferents, the laminar and regional organization of the inputs, and the relation of the tectum with primary and secondary visual and motor centers in goldfish were studied following HRP injections in the optic tectum, orbit of the eye, cerebellum, pretectal area, and dorsolateral mesencephalic tegmentum. Ipsilateral tectal afferents include the area dorsalis centralis of the forebrain, the nucleus dorsalis lateralis of the thalamus, the area pretectalis, the nucleus pretectalis, a nucleus in the rostral mesencephalic tegmentum, the torus longitudinales, the torus semicircularis, a dorsolateral tegmental nucleus, the nucleus isthmi, and a rostral cell group of the nucleus motorius tegmenti. Comparison of results in a series of tectal HRP injections which differed in depth, tangential extent, and location indicated that projections from the area pretectalis, nucleus pretectalis, and nucleus isthmi terminate in the stratum fibrosum et griseum superficiale of the tectum. Terminals of the forebrain and nucleus dorsolateralis and contralateral tectum are sparse and widely branching. Projections from the area and nucleus pretectalis tend to terminate in the rostral tectum, and those from the contralateral tectum, torus semicircularis, dorsolateral tegmental nucleus, and nucleus motorius tegmenti terminate preferentially in the caudal tectum. Cells of origin of extrinsic tectal efferents were also identified following HRP injections in the pretectal area and mesencephalic tegmentum. Proximal dendrites and axons of these cells were labeled sufficiently to allow comparison with morphological types characterized in Golgi studies. HRP injections in the cerebellum labeled cells bodies in the area pretectalis, nucleus pretectalis, and the nucleus of the posterior commissure. Double label experiments with intraocular injection of tritiated proline demonstrated direct retinal input to these three areas. No indications of direct connections between the tectum and cerebellum were found following tectal or cerebellar HRP injections.
在金鱼的视顶盖、眼眶、小脑、顶盖前区和背外侧中脑被盖区注射辣根过氧化物酶（HRP）后，研究了顶盖传入纤维的非视网膜来源、输入的分层和区域组织，以及顶盖与初级和次级视觉及运动中枢的关系。同侧顶盖传入纤维包括前脑的中央背侧区、丘脑的背外侧核、顶盖前区、顶盖前核、中脑嘴侧被盖中的一个核、纵隆起、半环形隆起、一个背外侧被盖核、峡核以及动眼神经被盖核的嘴侧细胞群。对一系列在深度、切线范围和位置上不同的视顶盖HRP注射结果进行比较表明，顶盖前区、顶盖前核和峡核的投射终止于视顶盖的浅纤维灰质层。前脑和背外侧核以及对侧顶盖的终末稀疏且分支广泛。顶盖前区和顶盖前核的投射倾向于终止于顶盖嘴侧，而对侧顶盖、半环形隆起、背外侧被盖核和动眼神经被盖核的投射则优先终止于顶盖尾侧。在顶盖前区和中脑被盖注射HRP后，也确定了顶盖外传出纤维的起源细胞。这些细胞的近端树突和轴突被充分标记，以便与高尔基研究中所描述的形态学类型进行比较。小脑注射HRP标记了顶盖前区、顶盖前核和后连合核中的细胞体。眼内注射氚化脯氨酸的双重标记实验证明视网膜直接输入到这三个区域。视顶盖或小脑注射HRP后，未发现视顶盖与小脑之间有直接连接的迹象。
