Robertson A J, Ramesar K C, Potts R C, Gibbs J H, Browning M C, Brown R A, Hayes P C, Beck J S
J Clin Lab Immunol. 1981 Jan;5(1):53-7.
Venous blood was removed from healthy subjects immediately before and at intervals after exercise on a bicycle ergometer. Immediately after exercise, the subjects developed a lymphocytosis in which (a) the E-rosette percentage fell by 10% but their absolute number in the circulating blood rose by more than 70%, (b) the percentage of PHA-stimulated cells fell, but their absolute numbers increased considerably and (c) the absolute numbers of Con A- and PWM-responding cells increased. The numbers of lymphocytes in the various subpopulations had returned virtually to the pre-exercise levels 15 minutes after stopping exercise. During the response to exercise, the characteristics of the cells in the various subpopulations did not show any major changes, despite considerable and rapid fluctuations in numbers: it is suggested that exercise discharges intravascularly sequestered cells and that cortisol may be involved in re-sequestration of stress-released cells. It is recommended that, in clinical studies on circulating lymphocyte numbers and populations, the patient should be rested for at least 15 minutes before venesection.
在健康受试者使用自行车测力计进行运动前及运动后的不同时间间隔，采集静脉血。运动后即刻，受试者出现淋巴细胞增多，表现为：(a) E花环百分比下降10%，但循环血液中其绝对数量增加超过70%；(b) PHA刺激细胞的百分比下降，但其绝对数量显著增加；(c) 对Con A和PWM反应的细胞绝对数量增加。停止运动15分钟后，各亚群淋巴细胞数量几乎恢复到运动前水平。在运动反应过程中，尽管细胞数量有相当大且快速的波动，但各亚群细胞的特征未显示出任何重大变化：提示运动使血管内隔离的细胞释放，且皮质醇可能参与应激释放细胞的再隔离。建议在关于循环淋巴细胞数量和亚群的临床研究中，患者在静脉采血前应至少休息15分钟。
