Jones P M, Robinson I C
J Neurosci Methods. 1981 Feb;3(3):295-300. doi: 10.1016/0165-0270(81)90065-0.
A new method is described for obtaining repeated cerebrospinal fluid (CSF) samples from conscious, unrestrained guinea pigs. A simple, disposable cannula is introduced into the cisterna magna by puncturing the atlanto-occipital membrane. The cannula is equipped with a silicone rubber disc which is glued to the membrane, forming a firm but flexible seal. Daily CSF samples of at least 100 microliters can be obtained from unrestrained guinea pigs. Chronically cannulated lactating females continued to suckle their litter successfully, and their milk ejection response was not affected by withdrawal of CSF.
描述了一种从清醒、不受束缚的豚鼠获取重复脑脊液（CSF）样本的新方法。通过穿刺枕寰膜将一个简单的一次性套管插入枕大池。该套管配有一个硅橡胶圆盘，圆盘粘贴在膜上，形成一个牢固但灵活的密封。每天可从不受束缚的豚鼠获取至少100微升的脑脊液样本。长期插管的哺乳期雌性豚鼠能够继续成功地哺育它们的幼崽，并且它们的喷乳反射不受脑脊液抽取的影响。
