Vistelle R, Wiczewski M, Aurousseau M
Laboratory of Pharmacology, Faculty of Pharmacy, Reims, France.
Brain Res Bull. 1989 May;22(5):919-22. doi: 10.1016/0361-9230(89)90038-5.
A cannulation technique for continuous sampling of cerebrospinal fluid (CSF) in unanesthetized freely moving rabbits is described. A permanent stainless steel cannula constructed easily and inexpensively from commercially available material is placed into the third ventricle and fixed to the skull by anchoring screws and dental cement. This procedure allows a continuous CSF sampling with a flow rate of 11.2 +/- 4.5 microliters/min without any sign of disturbing the animal. The penetration of the third ventricle or the periodic drainage of CSF, during a one month period, does not result in a variation of CSF protein (356 +/- 20 mg/l), glucose (4.5 +/- 0.2 mmol/l) or lactate (1.80 +/- 0.05 mmol/l) concentrations. Our technique has some advantages for pharmacological or pharmacokinetic investigations. The third ventricle CSF data will complete that of cisterna magna or lateral ventricle CSF. This method allows studies in the same animal during a period of one month without any problem. Moreover, it lends itself to experiments in which physiological conditions are required.
本文描述了一种用于在未麻醉的自由活动兔子中连续采集脑脊液（CSF）的插管技术。一种由市售材料轻松且廉价制成的永久性不锈钢插管被置入第三脑室，并通过固定螺钉和牙科水泥固定在颅骨上。该操作允许以11.2±4.5微升/分钟的流速连续采集脑脊液，且没有任何干扰动物的迹象。在一个月的时间里，第三脑室的穿刺或脑脊液的定期引流不会导致脑脊液蛋白（356±20毫克/升）、葡萄糖（4.5±0.2毫摩尔/升）或乳酸（1.80±0.05毫摩尔/升）浓度的变化。我们的技术在药理学或药代动力学研究方面具有一些优势。第三脑室的脑脊液数据将补充枕大池或侧脑室脑脊液的数据。该方法允许在同一动物身上进行为期一个月的研究而没有任何问题。此外，它适用于需要生理条件的实验。
