• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Binding of cytochrome b5 to membranes of isolated subcellular organelles from rat liver.细胞色素b5与大鼠肝脏分离的亚细胞细胞器膜的结合。
J Cell Biol. 1978 Nov;79(2 Pt 1):291-313. doi: 10.1083/jcb.79.2.291.
2
The binding of cytochrome b5 to plasma membranes of rat liver: its implication for membrane specificity and biogenesis.细胞色素b5与大鼠肝脏质膜的结合：其对膜特异性和生物发生的意义。
Biochim Biophys Acta. 1980 Apr 24;597(3):564-76. doi: 10.1016/0005-2736(80)90228-x.
3
Analytical study of microsomes and isolated subcellular membranes from rat liver. V. Immunological localization of cytochrome b5 by electron microscopy: methodology and application to various subcellular fractions.大鼠肝脏微粒体和分离的亚细胞膜的分析研究。V. 细胞色素b5的免疫电镜定位：方法及其在各种亚细胞组分中的应用
J Cell Biol. 1976 Nov;71(2):535-50. doi: 10.1083/jcb.71.2.535.
4
Localization and biosynthesis of NADH-cytochrome b5 reductase, an integral membrane protein, in rat liver cells. I. Distribution of the enzyme activity in microsomes, mitochondria, and golgi complex.大鼠肝细胞中整合膜蛋白NADH-细胞色素b5还原酶的定位与生物合成。I. 该酶活性在微粒体、线粒体和高尔基体中的分布
J Cell Biol. 1980 Jun;85(3):501-15. doi: 10.1083/jcb.85.3.501.
5
Intermembrane electron transport in the absence of added water-soluble carriers.
Biochim Biophys Acta. 1975 Nov 11;408(2):93-100. doi: 10.1016/0005-2728(75)90001-8.
6
Immunological similarity of the NADH-cytochrome c electron transport system in microsomes, Golgi complex and mitochondrial outer membrane of rat liver cells.
FEBS Lett. 1976 Apr 1;63(2):231-4. doi: 10.1016/0014-5793(76)80101-9.
7
Localization and biosynthesis of NADH-cytochrome b5 reductase, an integral membrane protein, in rat liver cells. II. Evidence that a single enzyme accounts for the activity in its various subcellular locations.大鼠肝细胞中整合膜蛋白NADH-细胞色素b5还原酶的定位与生物合成。II. 单一酶负责其在不同亚细胞位置活性的证据。
J Cell Biol. 1980 Jun;85(3):516-26. doi: 10.1083/jcb.85.3.516.
8
Cytochrome b5-like hemoprotein/cytochrome b5 reductase complex in rat liver mitochondria has NADH-linked aquacobalamin reductase activity.大鼠肝脏线粒体中的细胞色素b5样血红蛋白/细胞色素b5还原酶复合物具有与NADH相关的水钴胺素还原酶活性。
J Nutr. 1994 Jul;124(7):1037-40. doi: 10.1093/jn/124.7.1037.
9
Cytochrome b5-like hemoprotein of outer mitochondrial membrane: OM cytochrome b. II. Contribution of OM cytochrome b to rotenone-insensitive NADH-cytochrome c reductase activity.线粒体外膜细胞色素b5样血红素蛋白：线粒体外膜细胞色素b。II。线粒体外膜细胞色素b对鱼藤酮不敏感的NADH-细胞色素c还原酶活性的贡献。
J Biochem. 1980 Jan;87(1):73-80. doi: 10.1093/oxfordjournals.jbchem.a132754.
10
Binding of homogeneous cytochrome b5 to rat liver microsomes. Effect on N-demethylation reactions.同源细胞色素b5与大鼠肝脏微粒体的结合。对N-脱甲基反应的影响。
Biochim Biophys Acta. 1975 Nov 20;410(1):32-44. doi: 10.1016/0005-2744(75)90205-3.

引用本文的文献

1
Transmembrane topogenesis of a tail-anchored protein is modulated by membrane lipid composition.尾部锚定蛋白的跨膜拓扑形成受膜脂成分调控。
EMBO J. 2005 Jul 20;24(14):2533-42. doi: 10.1038/sj.emboj.7600730. Epub 2005 Jun 23.
2
Novel targeting signals mediate the sorting of different isoforms of the tail-anchored membrane protein cytochrome b5 to either endoplasmic reticulum or mitochondria.新型靶向信号介导尾锚定膜蛋白细胞色素b5的不同异构体分选至内质网或线粒体。
Plant Cell. 2004 Nov;16(11):3002-19. doi: 10.1105/tpc.104.026039. Epub 2004 Oct 14.
3
Synthesis of rat liver microsomal cytochrome b5 by free ribosomes.游离核糖体合成大鼠肝脏微粒体细胞色素b5 。
J Cell Biol. 1980 Mar;84(3):705-16. doi: 10.1083/jcb.84.3.705.
4
Absence of sugars in electrophoretically purified cytochrome b5 demonstrated by combined gas chromatography-mass spectrometry.气相色谱 - 质谱联用技术证明电泳纯化的细胞色素b5中无糖存在。
J Cell Biol. 1981 Jun;89(3):615-20. doi: 10.1083/jcb.89.3.615.
5
Peroxisomes in sebaceous glands. V. Complex peroxisomes in the mouse preputial gland: serial sectioning and three-dimensional reconstruction studies.皮脂腺中的过氧化物酶体。V. 小鼠包皮腺中的复杂过氧化物酶体：连续切片和三维重建研究。
Anat Embryol (Berl). 1984;169(3):261-70. doi: 10.1007/BF00315631.
6
Membranes of rat liver peroxisomes.大鼠肝脏过氧化物酶体的膜
Histochemistry. 1981;71(2):259-67. doi: 10.1007/BF00507829.
7
The carboxy-terminal 10 amino acid residues of cytochrome b5 are necessary for its targeting to the endoplasmic reticulum.细胞色素b5的羧基末端10个氨基酸残基是其靶向内质网所必需的。
EMBO J. 1992 Nov;11(11):4197-203. doi: 10.1002/j.1460-2075.1992.tb05513.x.
8
Membrane-bound redox proteins of the murine Friend virus-induced erythroleukemia cell.鼠类Friend病毒诱导的红白血病细胞的膜结合氧化还原蛋白。
J Cell Biol. 1979 Oct;83(1):231-9. doi: 10.1083/jcb.83.1.231.

本文引用的文献

1
A Hemochromogen Component of Liver Microsomes.肝微粒体的一种血色原成分。
Proc Natl Acad Sci U S A. 1952 Jan;38(1):19-25. doi: 10.1073/pnas.38.1.19.
2
The hydrolysis of rabbit y-globulin and antibodies with crystalline papain.用结晶木瓜蛋白酶对兔γ球蛋白和抗体进行水解。
Biochem J. 1959 Sep;73(1):119-26. doi: 10.1042/bj0730119.
3
Tissue fractionation studies. 4. Comparative study of the binding of acid phosphatase, beta-glucuronidase and cathepsin by rat-liver particles.组织分级分离研究。4. 大鼠肝脏颗粒对酸性磷酸酶、β-葡萄糖醛酸酶和组织蛋白酶结合的比较研究。
Biochem J. 1955 Mar;59(3):433-8. doi: 10.1042/bj0590433.
4
An electron-transport system associated with the outer membrane of liver mitochondria. A biochemical and morphological study.与肝线粒体外膜相关的电子传递系统。一项生化与形态学研究。
J Cell Biol. 1967 Feb;32(2):415-38. doi: 10.1083/jcb.32.2.415.
5
Intracellular transport of secretory proteins in the pancreatic exocrine cell. I. Role of the peripheral elements of the Golgi complex.胰腺外分泌细胞中分泌蛋白的细胞内运输。I. 高尔基体复合体周边元件的作用。
J Cell Biol. 1967 Aug;34(2):577-96. doi: 10.1083/jcb.34.2.577.
6
Biochemical analysis of the pinocytotic process. I. Isolation and chemical composition of the lysosomal and the plasma membrane of the rat liver cell.胞饮过程的生化分析。I. 大鼠肝细胞溶酶体和质膜的分离及化学成分
Hoppe Seylers Z Physiol Chem. 1970 Oct;351(10):1191-9. doi: 10.1515/bchm2.1970.351.2.1191.
7
The binding of cytochrome b5 to phosphatidylcholine vesicle.
Biochem Biophys Res Commun. 1973 Sep 18;54(2):808-15. doi: 10.1016/0006-291x(73)91496-4.
8
Microperoxisomes.微过氧化物酶体
J Histochem Cytochem. 1973 Nov;21(11):963-6. doi: 10.1177/21.11.963.
9
Proceedings: The binding of cytochrome b5 to liposomes.
Hoppe Seylers Z Physiol Chem. 1974 Oct;355(10):1238.
10
The binding of cytochrome b 5 to liver microsomes.细胞色素b5与肝微粒体的结合。
J Biol Chem. 1972 Nov 25;247(22):7188-94.

细胞色素b5与大鼠肝脏分离的亚细胞细胞器膜的结合。

Binding of cytochrome b5 to membranes of isolated subcellular organelles from rat liver.

作者信息

Remacle J

出版信息

J Cell Biol. 1978 Nov;79(2 Pt 1):291-313. doi: 10.1083/jcb.79.2.291.

DOI:10.1083/jcb.79.2.291
PMID:721892
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2110249/
Abstract

The in vitro incorporation of a well-characterized integral protein cytochrome b5 into membranes of various subcellular organelles was investigated by biochemical and immunochemical methods. Microsomes, peroxisomes, and outer mitochondrial membranes, all containing endogenous cytochrome b5, incorporated large amounts of the hemoprotein in such a way that it was reducible by an inherent NADH cytochrome b5 reductase. Lysosomal membranes did not incorporate cytochrome b5. Inner mitochondrial and Golgi membranes, which do not naturally contain cytochrome b5, bound it in vitro but it was not reduced in the presence of NADH. These results show some discrepancies between the natural localization and the in vitro binding of cytochrome b5. They confirm one aspect of the fluid membrane theory and bring new elements to our understanding of the maintenance of the specific features of the membranes of subcellular organelles with respect to the cell dynamism.

摘要

通过生化和免疫化学方法研究了一种特性明确的整合蛋白细胞色素b5在体外掺入各种亚细胞器膜的情况。微粒体、过氧化物酶体和线粒体外膜均含有内源性细胞色素b5，它们以能被内在的NADH细胞色素b5还原酶还原的方式掺入了大量的血红素蛋白。溶酶体膜不掺入细胞色素b5。线粒体内膜和高尔基体膜天然不含细胞色素b5，它们在体外能结合细胞色素b5，但在有NADH存在的情况下不能被还原。这些结果显示了细胞色素b5的天然定位和体外结合之间存在一些差异。它们证实了流体膜理论的一个方面，并为我们理解亚细胞器膜相对于细胞动态变化的特定特征的维持带来了新的因素。