Sahu S P
Am J Vet Res. 1981 Jan;42(1):45-8.
Pony mares were vaccinated with killed contagious equine metritis (CEM) bacteria by IV, subcutaneous, and intrauterine (IU) routes (or a combination of these routes). The serum agglutinating antibody titer varied from 1:64 to 1:1,024 after vaccination. In pony mares challenge exposed with 96-hour-old culture of CEM bacteria given by IU route, there were clinical signs of CEM, but these signs were less severe in vaccinated mares than in nonvaccinated mares. The bacterium was isolated for the exudate and from uterine samples collected from the mares after challenge exposure. A low titer of IU antibodies to CEM bacteria in infected mares was observed with agglutination tests (plate, tube, and antiglobulin), and enzyme-linked immunosorbent assay. However, a high antibody titer was obtained when passive hemagglutination test was used.
将孕马通过静脉注射、皮下注射和子宫内（IU）途径（或这些途径的组合）接种传染性马子宫炎（CEM）死菌疫苗。接种后血清凝集抗体效价在1:64至1:1024之间。在用IU途径给予的96小时龄CEM细菌培养物对孕马进行攻毒时，出现了CEM的临床症状，但接种疫苗的母马的这些症状比未接种疫苗的母马轻。从攻毒后母马的渗出物和子宫样本中分离出该细菌。通过凝集试验（平板、试管和抗球蛋白）和酶联免疫吸附测定观察到感染母马中针对CEM细菌的IU抗体效价较低。然而，当使用被动血凝试验时获得了高抗体效价。
