Hitchcock P J, Brown T M, Corwin D, Hayes S F, Olszewski A, Todd W J
Infect Immun. 1985 Apr;48(1):94-108. doi: 10.1128/iai.48.1.94-108.1985.
Examination of recently isolated cultures of three strains of Contagious Equine Metritis Organism grown on specially formulated, serum-free, clear typing medium revealed the presence of numerous colonial opacity variants. These colonies were prepared by a number of fixation and staining techniques and examined by scanning and transmission electron microscopy. Opaque and transparent phenotypes produced copious amounts of extracellular material compared with intermediate-opacity phenotypes which produced little or none. Also unique to intermediate colonies were numerous thin intercellular strands, which may represent pili or polymers of extracellular material. The presence of an unusual fibrillar layer (with similar electron density to the extracellular material) on the outer leaf of the outer membrane also was confirmed. A number of other ultrastructural features also were noted, including an epilayer, a thin nonmembranous layer which covered colonies and adjacent agar.
对在特殊配制的无血清透明分型培养基上培养的三株马传染性子宫炎病原体的近期分离培养物进行检查，发现存在大量菌落不透明度变异体。这些菌落通过多种固定和染色技术制备，并通过扫描电子显微镜和透射电子显微镜进行检查。与产生少量或不产生细胞外物质的中间不透明度表型相比，不透明和透明表型产生大量细胞外物质。中间菌落还特有的是大量细的细胞间链，这可能代表菌毛或细胞外物质的聚合物。外膜外叶上存在异常的纤维层（电子密度与细胞外物质相似）也得到了证实。还注意到许多其他超微结构特征，包括一个覆盖菌落和相邻琼脂的表层，即一层薄的非膜性层。
