Hunt J S, Jackson A E, Day W A, McGiven A R
Br J Exp Pathol. 1981 Feb;62(1):52-8.
A method is described for the culture of isolated intact mouse glomeruli. Cultures showed adherent glomeruli after 2 days and from 2-4 days a monolayer of cells began to develop around the points of adherence. The predominant cell (Type I) was 80-200 micrometer in diameter with cytoplasmic extensions, later becoming polyhedral and often multinucleated. Type II spindle cells, 70-100 micrometer in length, were less prominent in the earlier stages and later formed sheaves between Type I cells. Small phagocytic Type III cells, 10-20 micrometer in diameter, were not prominent in cultures from normal kidneys and tended to form small clusters on top of the Type I cell monolayer.
本文描述了一种分离完整小鼠肾小球的培养方法。培养2天后可见贴壁的肾小球，在2至4天内，围绕附着点开始形成单层细胞。主要细胞（I型）直径为80 - 200微米，有细胞质突起，随后变为多面体，且常为多核。II型梭形细胞长70 - 100微米，在早期不太明显，后期在I型细胞之间形成束状。III型小吞噬细胞直径为10 - 20微米，在正常肾脏来源的培养物中不突出，倾向于在I型细胞单层顶部形成小簇。
