Rivedal E, Sanner T
Cancer Lett. 1980 Jan;8(3):203-8. doi: 10.1016/0304-3835(80)90002-6.
Morphological transformation and induction of somatic mutation in the hamster embryo cell bioassay have been used to study whether the carcinogenicity of nickel is affected by polycyclic hydrocarbons. The transformation frequency was found to increase with increasing concentration of nickel sulphate, benz[a]pyrene (BP) and methylcholanthrene. In experiments with combinations of nickel sulphate and BP, the transformation frequencies used for all concentrations were higher than for compounds tested separately. The greatest enhancement was found using 5 micrograms/ml NiSO4 . 6H2O and 0.78 microgram/ml BP. The transformation frequency obtained with this combination was 10.7%, compared to 0.5% and 0.6% for the individual substances. No synergistic effect could be detected between nickel sulphate and methylcholanthrene (MC). In experiments measuring somatic mutation by selection for ouabain resistance, the mutation frequency was likewise found to be significantly higher than expected in mixtures of nickel sulphate and BP. The present demonstration of the synergistic effect between nickel sulphate and BP is of interest with the potentiating effect of cigarette smoking on development of lung cancer among nickel refinery workers.
在仓鼠胚胎细胞生物测定中，利用形态学转化和体细胞突变诱导来研究镍的致癌性是否受多环烃影响。发现转化频率随硫酸镍、苯并[a]芘（BP）和甲基胆蒽浓度的增加而升高。在硫酸镍和BP组合的实验中，所有浓度下的转化频率均高于单独测试的化合物。使用5微克/毫升硫酸镍·6水合物和0.78微克/毫升BP时增强作用最大。该组合获得的转化频率为10.7%，而单个物质分别为0.5%和0.6%。在硫酸镍和甲基胆蒽（MC）之间未检测到协同效应。在通过选择哇巴因抗性来测量体细胞突变的实验中，同样发现硫酸镍和BP混合物中的突变频率显著高于预期。硫酸镍和BP之间协同效应的当前证明对于吸烟对镍精炼厂工人肺癌发生的增强作用具有重要意义。
