Covalent binding of chloroform metabolites to nuclear proteins - no evidence for binding to nucleic acids.

No evidence was found for covalent binding of CHCl3 metabolites to male Sprague-Dawley rats or strain A/J male mice liver DNA or RNA under different experimental conditions including phenobarbital or 3-methyl-cholanthrene or diethylmaleate treatments or multiple CHCl3 injections. In addition, no binding to DNA was observed when DNA was exposed to CHCl3 in incubation mixtures containing microsomes and NADPH or when tissue slices were incubated with CHCl3. In contrast, nuclear histone and non-histone proteins bound to CHCl3 metabolites.