Dell'Orco R T, Anderson L E
Cell Biol Int Rep. 1981 Apr;5(4):359-64. doi: 10.1016/0309-1651(81)90005-9.
Human diploid fibroblast-like cells from two donor of widely different ages were tested for their ability to perform unscheduled DNA synthesis (UDS) after exposure to ultraviolet irradiation. Cultures were assayed at various times throughout their in vitro lifespans as both confluent and mitotically arrested populations. Cells from both donors maintained their ability to perform UDS throughout their lifespans with arrested populations exhibiting increased levels. The appearance of elevated levels of UDS in arrested cells was directly related to the age of the donor. These results indicate that the loss of ability to repair DNA damage is not a primary cause of in vitro senescence. They do suggest, however, that the regulation of DNA repair synthesis is effected by increasing age.
对来自两个年龄差异很大的供体的人二倍体成纤维样细胞进行了检测，以观察其在紫外线照射后进行非预定DNA合成（UDS）的能力。在整个体外寿命期间的不同时间，对汇合和有丝分裂停滞的细胞群体进行了测定。两个供体的细胞在其整个寿命期间都保持了进行UDS的能力，有丝分裂停滞的细胞群体表现出更高的水平。停滞细胞中UDS水平升高的出现与供体年龄直接相关。这些结果表明，修复DNA损伤能力的丧失不是体外衰老的主要原因。然而，它们确实表明，DNA修复合成的调节受年龄增长的影响。
